Method of controlling bacteria with trichloroacrylonitrile

ABSTRACT

This invention relates to a method of controlling bacteria by applying an effective amount of trichloroacrylonitrile to the habitat thereof.

United States Patent 1 Jan. 16, 1973 Baker METHOD OF CONTROLLING [56] References Cited BACTERIA WITH TRICHLOROACRYLONITRILE UNITED STATES PATENTS 3, I45, 1 38 8/ i 964 Baker 6! 3|. ..424/3()4 Inventor: Assignee: Stauffer Chemical Co., New York,

Filed: May 26, 1971 Appl. No.: 147,195

US. Cl ..424/304, 71/67 Int. Cl. ..A0ln 9/20 Field of Search ..424/304 Don Rjalter, Grind a, Calif. m

[57] ABSTRACT This invention relates to a method of controlling bacteria by applying an effective amount of trichloroacrylonitrile to the habitat thereof.

1 Claim, No Drawings METHOD OF CONTROLLING BACTERIA WITII T RICIILOROACRYLONITRILE DESCRIPTION OF THE INVENTION This invention relates to the novel use of trichloroacrylonitrile. More particularly, the present invention involves the novel use of trichloroacrylonitrile as a bactericide and algaecide.

As a composition of matter, trichloroacrylonitrile is known and described in prior art and its method of preparation is likewise adequately described. Moreover, its use as a fungicide and nematocide is described in U.S. Pat. No. 3,145,138. However, there is no disclosure of the novel use of the subject compound as described herein.

The following tests clearly demonstrate the activity of trichloroacrylonitrile.

Test 1 Algaecide and Bactericide Test This test measures the bactericidal and algicidal properties of a compound when in contact with a growing bacterium or algae in an artificial medium. The test is conducted by adding 20 ml. portions of a suitable warm steril agar solution into 20 X I mm. petri dishes. Next, the test compound (0.5 percent in acetone) is added to the petri dishes at levels of l, 5, l0 and 50 ug/ml and mixed with the warm mobile agar solution. The treated agar mixture is then allowed to cool to room temperature and solidify. Cells of the particular organism are then streaked on the surface of the solidified agar and then incubated for such lengths of time that the untreated samples containing no toxicant show luxurious growth typical of the particular organ ism. This time varies from 24 hours to 1 week, depending upon the particular organism. The bacteria are incubated at 30 to 37C. and the algae at room temperature under artificial light. Nutrient agar is used as the medium for the bacteria and a modified Jack Meyers solution plus agar is used as the medium for the algae. The table below shows the results when trichloroacrylonitrile are run in this test.

Concentration Necesary Bacteria for Control (pg/ml) Pseudomonas aeruginosa ID) a. Bacillus cereus 50 b.

Algae Scenedesmus obliquus (50) Chlorella pyrinoidosa 50 indicates partial control at this concentration.

a. incubated at 37C.

b. incubated at 30C.

Test 2 Sulfate Reducing Bactericidal Test This test measures the bactericidal effectiveness of a compound against sulfate reducing bacteria when grown in an artificial medium. The test is conducted using steril vials containing 9.0 ml. sulfate API broth with tryptone under anaerobic conditions. To these vials are added the test compound (0.5 percent in acetone) at levels to give I, 5, l0 and 50 ug/ml final concentration. Next, to the vials are added 0.4 ml. steril 

